ssDNA Quantification Kit (Half-Volume 100 µl Assay)

Assay tube adaptor and 100-µL assay tubes required.

BioDynami has developed Qubit assays with a volume of 100 µL. This volume is half of the standard assay (200 µL), and reagent costs are reduced by 50% compared to the 200 µL assay. The ssDNA kits can save 67% (200 µL assay) and 83% (half-volume 100 µL assay) of costs compared to ThermoFisher Qubit ssDNA kits.

Description: 

BioDynami DNA/RNA quantification kits are designed for accurate quantification of DNA and RNA samples across a wide concentration range using the Qubit™ fluorometer.

The ssDNA Quantification Kit is developed for quantification of single-stranded DNA and includes dye, dilution buffer, and two ssDNA standards. The assay is accurate for DNA concentrations ranging from 50 pg/µL to 200 ng/µL, based on comparison of the data with standards, as shown in the graph below.

The kit detects ssDNA using a fluorescent dye, which enables sensitive quantification of single-stranded DNA, including ssDNA viruses, synthetic ssDNA, the first stage of cDNA synthesis, denatured DNA, and bisulfite-converted DNA, etc.

Protocol:

The test procedure is simple – just dilute the ssDNA dye with the ssDNA dilution buffer, add the DNA sample (volume 1-20 μL), and then measure the concentration using the Qubit™ fluorometer.

Features:

• Optimized for use with the Qubit™ fluorometer
• Uses the Qubit™ ssDNA assay setting
• Linear range: 1–200 ng dsDNA
• Cost-effective – reduces costs by 50%

Effect of Contaminants:

The tests are well-tolerated by common contaminants such as salt, solvents and detergents as shown in the table below.

Qubit™ is a registered trademark of Thermo Fisher Scientific.