XChange Plasmid Midi Kit

PRODUCT HIGHLIGHTS
- Quick and easy protocol
- Isolation of highly pure plasmid DNA
from 5 to 100 ml overnight culture
- Short hands-on and preparation times
of only 65 min
- Clarifying of the lysate by
special pre-filters
- Plasmids efficiently bind to positively
charged methylethylamine groups
- High flow rates and short
precipitation times
- No need for centrifugation steps
effectively protects DNA during
extraction
- Complete enzymatic degradation
of all cellular nucleases
- Complete enzymatic degradation
of all cellular RNA
- Efficient removal of enzyme
inhibitors and contaminants
- Plasmid DNA is suitable for high
transfection efficiencies
PRINCIPLE
The peqGOLD XChange Plasmid Midi kits
combine a modified method of alkaline
lysis with the DNA binding properties of
XChange Anion exchange gels.
An optimized packing process leads to
a porous column matrix where bacterial
lysates or Wash and Elution buffers can
rapidly and consistently pass through by
gravitation. The column matrix contains
positively charged active groups which
bind the negatively charged DNA while
enzyme inhibitors and contaminants
are easily washed through. After the
washing step the pure plasmid DNA is
eluted and then alcohol precipitated.
Although flow rates are rapid and protocol
times are short, the isolated DNA
is highly pure, provides excellent chromatographic
definition and thus is ready
to use for a wide range of downstream
applications.