![https://www.carolinabiosystems.cz/282-thickbox_default/2x-bluestar-pcr-master-mix-100-reactions-50-l-vol.jpg](https://www.carolinabiosystems.cz/282-large_default/2x-bluestar-pcr-master-mix-100-reactions-50-l-vol.jpg)
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Recommended Storage Condition: Room temperature
PCR conditions:
1. Prepare the reaction at room temperature.
2. Add template, primers and H2O to make up ½ of the desired total volume, and then add equal volume of 2x master mix.
3. The master mix contains a high fidelity DNA polymerase.
4. The elongation speed of this master mix is about 2 Kb per minute.
5. The blue dye runs at about 1 Kb position on an agarose gel.