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2X BlueStar™ PCR Master Mix

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100 reactions (50 μl vol)

2x BlueStarTM PCR Master Mix contains all the necessary reagents and the gel loading dye. The PCR mix can be directly loaded to gel.

Recommended Storage Condition: Room temperature

PCR conditions:
1. Prepare the reaction at room temperature.
2. Add template, primers and H2O to make up ½ of the desired total volume, and then add equal volume of 2x master mix.
3. The master mix contains a high fidelity DNA polymerase.
4. The elongation speed of this master mix is about 2 Kb per minute.
5. The blue dye runs at about 1 Kb position on an agarose gel.


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