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- Nucleic Acid Analysis
- Protein Analysis
- Biochemical Reagents
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Enzymes
- Thermophilic DNA Polymerases
- Mesophilic DNA Polymerases
- Restriction Endonucleases
- Reverse Transcriptase and RNA Polymerases
- DNA/RNA Ligases
- RNases
- Proteases
- Nucleases
- Kinases
- Phosphatases and Sulfurylases
- DNA Repair Proteins
- Single-Stranded DNA Binding Proteins
- Chaperon Proteins and Disulfide Bond Isomerase
- Others
- Gene editing
- Molecular cloning
- Clinical diagnostics
- Human Identification STR kits
- Laboratory instruments
- Software
- A&A Biotechnology
News
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1st Czechoslovak Congress of Medical Genetics 2025
In the spring, we will participate in the 1st Czechoslovak Congress of Medical Genetics, which will take place from April 2–4, 2025, at the Cultural and Congress Center Elektra in the spa town of Luha...
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RANK 2025
Visit us at the 19th edition of the RANK 2025 conference, which will take place on March 19th and 20th at the Zlatá Štika Hotel in Pardubice. The conference is organized by the Czech Society of Clinic...
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Kapras Day 2025
We would like to invite you to the 23rd Kapras Day on the topic of "Clinical Genetics," which will take place on Wednesday, February 26, 2025, in the Congress Hall of Hotel Olšanka in Prague. We loo...
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Description:
MCLAB's BDX64 is a BigDye® enhancing buffer, it is used for DNA sequencing. BDX64 buffer has the same ion strength as the BigDye® premix 3.1 and 1.1.
Up to 64 (0.12 µl bigdye in 10µl reaction) or more fold dilutions by combining with the 5x dilution buffer
Enhances the polymerase activity and reduces the extension time from 4 to 1 minute.
Reduces the signal decline rate and results in even peak distributions.
Optimizes for using with BigDye® Chemistry (ABI) on 310, 3100, 3130/3130xl & 3730/3730xl.
Application:
|
Component |
Volume |
Volume |
Volume |
|
BigDye® 3.1 |
0.125 µl |
0.25 µl |
0.5 µl |
|
5x buffer (catalog#: SBUF-100) |
0.9375 µl |
0.875 µl |
0.75 µl |
|
BDX64 buffer |
2 µl |
2 µl |
2 µl |
|
Template |
100 ng |
100 ng |
100 ng |
|
Primer |
3.2pmol |
3.2pmol |
3.2pmol |
|
Add H2O to 10 µl |
|
|
|
Sequencing Examples:
1. Dilution fold 32X:
2. Dilution fold 64X:
3. Dilution fold 128X:
4. Dilution fold 256X:
Signal Distributions: before and after BDX64 was added
Recommended Cycle Conditions:
96°C, 3 minutes. + 30x (96°C, 10 seconds. 50°C, 5 seconds. 60°C, 2 minutes.)
Recommended Storage Condition: -20°C
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