BDX64 (BigDye® Enhancing Buffer)

Reference: BDX-100

2 x1.25 ml

More info

Description:
MCLAB's BDX64 is a BigDye^® enhancing buffer, it is used for DNA sequencing. BDX64 buffer has the same ion strength as the BigDye^® premix 3.1 and 1.1.
Up to 64 (0.12 µl bigdye in 10µl reaction) or more fold dilutions by combining with the 5x dilution buffer
Enhances the polymerase activity and reduces the extension time from 4 to 1 minute.
Reduces the signal decline rate and results in even peak distributions.
Optimizes for using with BigDye^® Chemistry (ABI) on 310, 3100, 3130/3130xl & 3730/3730xl.

Application:

Component	Volume	Volume	Volume
BigDye® 3.1	0.125 µl	0.25 µl	0.5 µl
5x buffer (catalog#: SBUF-100)	0.9375 µl	0.875 µl	0.75 µl
BDX64 buffer	2 µl	2 µl	2 µl
Template	100 ng	100 ng	100 ng
Primer	3.2pmol	3.2pmol	3.2pmol
Add H₂O to 10 µl

Sequencing Examples:
1. Dilution fold 32X:

2. Dilution fold 64X:

3. Dilution fold 128X:

4. Dilution fold 256X:

Signal Distributions: before and after BDX64 was added

Recommended Cycle Conditions:
96°C, 3 minutes. + 30x (96°C, 10 seconds. 50°C, 5 seconds. 60°C, 2 minutes.)

Recommended Storage Condition: -20°C