DNA Polymerase I is a DNA-dependent DNA polymerase. The enzyme catalyzes 5' -> 3' synthesis of DNA and exhibits 3' -> 5’ exonuclease proofreading activity.
DNA Polymerase I is a mesophilic DNA polymerase that exhibits 5' -> 3' DNA synthesis activity in addition to both 3' -> 5' and 5' -> 3' exonuclease activities. The combination of DNA synthesis and 5' -> 3' nuclease characteristics enable nick-translation during DNA synthesis.
- Nick translation of DNA to obtain probes with a high specific activity
- Second strand synthesis of cDNA
A recombinant E. coli strain carrying the PolA gene.
Specific Activity: 6,850U/mg
25 mM Tris-HCl
0.1 mM EDTA
1.0 mM DTTl
pH 7.4 @ 25°C
10x Blue Buffer
10x Blue Buffer:
500 mM NaCl
100 mM Tris-HCl
100 mM MgCl2
10 mM DTT
pH 7.9 @ 25°C
1 unit is defined as the amount of polymerase required to convert 10 nmol of dNTPs into acid insoluble material in 30 minutes at 37°C.
Recommended Storage Condition: -20°C