T7 DNA Polymerase is the mesophilic, highly processive, and replicative DNA polymerase from bacteriophage T7. It is responsible for the rapid and accurate replication of the virus genome during its infection cycle. T7 DNA Polymerase is a two subunit protein that consists of a polymerase domain (gene 5 from the T7 bacteriophage) and a processivity factor (E. coli trxA gene thioredoxin) (1, 2). The enzyme possesses a powerful (3' -> 5') nuclease activity that acts on both single and double stranded DNA and appears to be responsible for the high fidelity of this enzyme and prevents strand displacement synthesis(3,4,5).
Specific Activity: 10,000U/mg
Second strand synthesis in site-directed mutagenesis protocols
A recombinant E. coli strain carrying the bacteriophage T7 gene 5.
1 unit is defined as the amount of polymerase required to convert 10 nmol of total dNTPs into acid insoluble material in 30 minutes at 37°C.
50 mM KPO4
0.1 mM EDTA
1.0 mM Dithiothreitol
pH 7.0 @ 25°C
10x T7 DNA Polymerase Buffer
10x T7 DNA Polymerase Buffer:
400 mM Tris-HCl
200 mM MgCl2
500 mM NaCl
pH 7.5 @ 25°C
Recommended Storage Condition: -20°C
1. Grippo, P. et al. (1971) J. Biol. Chem. 246, 6867-6873
2. Modrich, P. et al. (1975) J. Biol. Chem. 250, 5515-5522
3. Adler, S. et al. (1979) J. Biol. Chem. 254, 11605-11614
4. Hori, K., et al. (1979) J. Biol. Chem. 254, 11598-11604
5. Lechner, R. L. et al. (1983) J. Biol. Chem. 258, 11185-11196