Description: A recombinant E. coli strain carrying the BST DNA Polymerase gene (large fragment).
Figure: MCLAB Bst DNA Polymerase (large fragment) shows Higher strand -displacing polymerase activity than other two vendors' products.
Application: - Sequencing through problematic secondary structures
Source: Bst DNA Polymerase (large fragment) is the portion of the Bacillus stearothermophilus DNA Polymerase protein that contains the 5´-> 3´ polymerase activity, but lacks the 5'->3' exonuclease domain.
Specific Activity: 120,000 U/mg
Supplied in: 10 mM Tris-HCl 50 mM KCl 1.0 mM DTT 0.1 mM EDTA 0.1% Triton X-100 50% Glycerol pH 7.5 @ 25°C
Supplied with: 10X PCR buffer II
10x PCR Buffer ll: 200 mM Tris-HCl 100 mM Ammonium Sulfate 100 mM KCl 20 mM MgSO4 1.0% Triton X-100 pH 8.8 @ 25°C
Unit Definition: 1 unit is defined as the amount of polymerase required to convert 10 nmol of dNTPs into acid insoluble material in 30 minutes at 65°C.
Recommended Storage Condition: -20°C
Reference: Kiefer, et al. Structure 15 January 1997. 5, 95-108.