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Bst DNA Polymerase (large fragment)

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For DNA sequencing high GC regions and rapid sequencing from nanogram amounts of DNA templates.

A recombinant E. coli strain carrying the BST DNA Polymerase gene (large fragment).

Figure: MCLAB Bst DNA Polymerase (large fragment) shows Higher strand -displacing polymerase activity than other two vendors' products.

- Sequencing through problematic secondary structures

Bst DNA Polymerase (large fragment) is the portion of the Bacillus stearothermophilus DNA Polymerase protein that contains the 5´-> 3´ polymerase activity, but lacks the 5'->3' exonuclease domain.

Specific Activity: 120,000 U/mg

Supplied in:
10 mM Tris-HCl
50 mM KCl
1.0 mM DTT
0.1 mM EDTA
0.1% Triton X-100
50% Glycerol
pH 7.5 @ 25°C

Supplied with:
10X PCR buffer II

10x PCR Buffer ll: 
200 mM Tris-HCl
100 mM Ammonium Sulfate
100 mM KCl
20 mM MgSO4
1.0% Triton X-100
pH 8.8 @ 25°C

Unit Definition:
1 unit is defined as the amount of polymerase required to convert 10 nmol of dNTPs into acid insoluble material in 30 minutes at 65°C.

Recommended Storage Condition: -20°C

Kiefer, et al. Structure 15 January 1997. 5, 95-108.


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