Inorganic Pyrophosphatase, E. coli

Description:
Inorganic pyrophosphatase (PPase) is ubiquitous in nature and plays an important role in energy metabolism, providing a thermodynamic pull for biosynthetic reactions such as protein, RNA, and DNA synthesis. Escherichia coli K-12 gene ppa encoding inorganic pyrophosphatase (PPase) was cloned and sequenced. The 5'-end of the ppa mRNA was identified by primer extension mapping.

Source:
E. coli strain carrying a plasmid encoding pyrophosphatase from E. coli K-12.

Application:
- Role in protein, RNA, and DNA synthesis
- Catalyzing the reaction PPi + H₂O —> 2P_i

Unit Definition:
One unit will release 1.0 µmole of inorganic orthophosphate per minute at pH 8.5 at 37°C.

Specific Activity:  3,500 U/mg

Recommended Storage Condition: -20°C

Reference:
Kornberg, A. 1962. On the metabolic significance of phosphorolytic and pyrophosphorolytic reactions, p. 251-264. In H. Kasha and P. Pullman (ed.), Horizons in biochemistry. Academic Press, New York.