HindIII

Recognition site: A^AGCTT

Source:
Purified from a strain of E. coli that bears the cloned Hind III gene.

Storage Buffer:
10 mM Tris-HCl (pH 7.4)
200 mM NaCl
0.5 mM EDTA
1mM DTT
0.5 mg/ml BSA
50% (v/v) Glycerol

10x Hind III Buffer
50 mM NaCl
10 mM Tris-HCl
10 mM MgCl₂
100 μg/ml BSA
pH 7.9 @ 25°C

Reaction Conditions:
Buffer at 37°C.
Typical Ligation/Recut Assay Results:
Hind III-cleaved λ DNA is ≥95% ligated after 1 h. Of the ligated fragments, 100% are cleaved (recut) by Hind III.

Recommended Storage Condition: -20°C.

Comments:
Does not cleave DNA when either the 5'A or the C residue is N6 –methyladenine or 5-methylcytosine, respectively. Resistant to heat inactivation (10 minutes, 65°C).