Poly(A) Polymerase, E. coli.

Description:
Poly(A) Polymerase catalyzes the addition of AMP from ATP to the 3'-hydroxyl of RNA. The reaction requires Mg²⁺ and is template independent.

Application:
- Labeling of RNA with ATP or cordycepin
- Poly(A) tailing of RNA for cloning or affinity purification
- Enhances translation of RNA transferred into eukaryotic cells

Source:
The gene encoding E. coli Poly(A) Polymerase expressed in E. coli.

Supplied in:
25 mM Tris-HCl
500 mM NaCl
1 mM MgCl₂
0.1 mM DTT
0.1 mM EDTA
50% Glycerol
pH 8.0 @ 25°C

Supplied with:
10x Poly(A) Polymerase Reaction Buffer

10x Poly(A) Polymerase Reaction Buffer:
500 mM Tris-HCl
2.5 M NaCl
100 mM MgCl₂
pH 7.9 @ 25°C

Unit Definition:
1 unit is defined as the amount of enzyme that will incorporate 1 nmol of ATP into acid-insoluble material in 10 minutes at 37°C.

Recommended Storage Condition: -20°C