BrightDye® Terminator v3.1 Cycle Sequencing Kit

Description:
The BrightDye® Terminator Cycle Sequencing Kit is designed for de novo sequencing, resequencing,  PCR product, plasmid, fosmid, and BAC templates by utilizing highly flexible chemistry. This kit uses four different fluorescent dyes to label ddNTPs, which are added sequentially to the primer through a cycle sequencing reaction. All required reagents for the sequencing procedure are in a reaction-ready, pre-mixed format. BrightDye terminator kit is compatible with commercially available BigDye terminator V3.1 kit, without any changes in the workflow.
With enhanced robustness and better dye mobility characteristics, the BrightDye® Terminator Cycle Sequencing Kit can be used in a wide range of applications with the following features:

• -Long read lengths.
• -Better performance reading through GC rich regions.
• -Longer reads with more uniform peak heights.
• -Improved productivity for less cost.

Applications:
De novo sequencing
Resequencing
Sequencing difficult templates Long-read sequencing
Sequencing across all template types (plasmids, PCR products, BACs, and fosmids)
Mixed-base detection
Sequencing short PCR products using rapid electrophoresis run modules

Platforms:
GeneAmp® 9700, Veriti® Thermal Cycler, 3730 DNA Analyzer, 3500xL Genetic Analyzer, 3500 Genetic Analyzer, 310 Genetic Analyzer, 3130 Genetic Analyzer, 3130xl Genetic Analyzer, 3730xl DNA Analyzer

Template Compatibility:
Plasmid DNA (≤15Kb)
Fosmids
Single Stranded DNA
Lambda DNA
BAC DNA
Genomic DNA (Bacterial)
PCR Amplicons

Storage:
Store kit at -20°C. Avoid repeated freeze-thaw cycles.

Regulatory Statement:
For Research Use Only. Not for use in diagnostic procedures.