Description:
Chemically competent E. coli cells suitable for transformation and protein expression. This strain does not express the T7 RNA polymerase.
Application:
Recommended torage condition:
This product should be stored at -80°C. Thaw on ice only before use. Do not re-freeze.
Recommended reaction conditions:
Genotype:
E. coli B F- dcm ompT hsdS(rB- mB-) gal [malB+]K-12(λS)
Reference:
Miroux B, Walker JE. J. Mol. Biol. (1996) 260, 289-298.
Competent Cell Type:
Chemically
Transformation Efficiency (cfu/μg):
>1x108
Blue-Whiet Screening:
No
Strain:
B
Reduces Recombination:
No
Cloning Methylated DNA:
No
Improves Plasmid Quality:
No
Preparing Unmethylated DNA:
Not suitable
T1 Phage Resistant:
No
RecA Deficient:
No