BL21 Competent E. coli

Description:
Chemically competent E. coli cells suitable for transformation and protein expression. This strain does not express the T7 RNA polymerase.

Application:

• Ideal for expressing proteins in E. coli.

Recommended torage condition:
This product should be stored at -80°C. Thaw on ice only before use. Do not re-freeze.

Recommended reaction conditions:

• Mix DNA with competent cells
• Let stand on bench for 5 minutes
• Direct plate

Genotype:
E. coli B F- dcm ompT hsdS(rB- mB-) gal [malB+]K-12(λS)

Reference:
Miroux B, Walker JE. J. Mol. Biol. (1996) 260, 289-298.

Competent Cell Type:
Chemically

Transformation Efficiency (cfu/μg): 
>1x10⁸

Blue-Whiet Screening:
No

Strain:
B

Reduces Recombination:
No

Cloning Methylated DNA:
No

Improves Plasmid Quality:
No

Preparing Unmethylated DNA:
Not suitable

T1 Phage Resistant:
No

RecA Deficient:
No