Description
High yields, high efficiency, and no heat shock necessary.
Application
Recommended torage condition
This product should be stored at -80°C. Thaw on ice only before use. Do not re-freeze.
Recommended reaction conditions
Genotype
F– ompT gal dcm lon hsdSB(rB- mB-) λ(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5])
Reference
Miroux B, Walker JE. J. Mol. Biol. (1996) 260, 289-298.
Competent Cell Type:
Chemically
Transformation Efficiency (cfu/μg):
>1x108
Blue-Whiet Screening:
No
Strain:
B
Reduces Recombination:
No
Cloning Methylated DNA:
No
Improves Plasmid Quality:
No
Preparing Unmethylated DNA:
Not suitable
T1 Phage Resistant:
No
RecA Deficient:
No