View full size
- Nucleic Acid Analysis
- Protein Analysis
- Biochemical Reagents
-
Enzymes
- Thermophilic DNA Polymerases
- Mesophilic DNA Polymerases
- Restriction Endonucleases
- Reverse Transcriptase and RNA Polymerases
- DNA/RNA Ligases
- RNases
- Proteases
- Nucleases
- Kinases
- Phosphatases and Sulfurylases
- DNA Repair Proteins
- Single-Stranded DNA Binding Proteins
- Chaperon Proteins and Disulfide Bond Isomerase
- Others
- Gene editing
- Molecular cloning
- Clinical diagnostics
- Human Identification STR kits
- Laboratory instruments
- Software
- A&A Biotechnology
- AdvancedSeq
- BioDynami
- Plant Cell Technology
News
-
RANK 2026
Visit us at the jubilee 20th edition of the RANK 2026 professional conference, which will take place on March 18–19 in Pardubice at the ABC Club. The conference is organized by the Czech Society of Cl...
Read more -
XXXV. Izakovičov memoriál 2025
We are pleased to announce our participation in the prestigious XXXV. Izakovič Memorial 2025, which will take place on October 8–10, 2025 at the Grandhotel Praha, Tatranská Lomnica. The Izakovič Memo...
Read more -
1st Czechoslovak Congress of Medical Genetics 2025
In the spring, we will participate in the 1st Czechoslovak Congress of Medical Genetics, which will take place from April 2–4, 2025, at the Cultural and Congress Center Elektra in the spa town of Luha...
Read more
View full size
Description
High yields, high efficiency, and no heat shock necessary.
Application
- Ideal for expressing proteins in E. coli.
- BL21(DE3) cells carry the lambda DE3 lysogen.
Recommended torage condition
This product should be stored at -80°C. Thaw on ice only before use. Do not re-freeze.
Recommended reaction conditions
- Mix DNA with competent cells
- Let stand on bench for 5 minutes
- Direct plate
Genotype
F– ompT gal dcm lon hsdSB(rB- mB-) λ(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5])
Reference
Miroux B, Walker JE. J. Mol. Biol. (1996) 260, 289-298.
Competent Cell Type:
Chemically
Transformation Efficiency (cfu/μg):
>1x108
Blue-Whiet Screening:
No
Strain:
B
Reduces Recombination:
No
Cloning Methylated DNA:
No
Improves Plasmid Quality:
No
Preparing Unmethylated DNA:
Not suitable
T1 Phage Resistant:
No
RecA Deficient:
No
Cart
Product
(empty)
Payment gate
