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- Nucleic Acid Analysis
- Protein Analysis
- Biochemical Reagents
-
Enzymes
- Thermophilic DNA Polymerases
- Mesophilic DNA Polymerases
- Restriction Endonucleases
- Reverse Transcriptase and RNA Polymerases
- DNA/RNA Ligases
- RNases
- Proteases
- Nucleases
- Kinases
- Phosphatases and Sulfurylases
- DNA Repair Proteins
- Single-Stranded DNA Binding Proteins
- Chaperon Proteins and Disulfide Bond Isomerase
- Others
- Gene editing
- Molecular cloning
- Clinical diagnostics
- Human Identification STR kits
- Laboratory instruments
- Software
- A&A Biotechnology
News
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1st Czechoslovak Congress of Medical Genetics 2025
In the spring, we will participate in the 1st Czechoslovak Congress of Medical Genetics, which will take place from April 2–4, 2025, at the Cultural and Congress Center Elektra in the spa town of Luha...
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RANK 2025
Visit us at the 19th edition of the RANK 2025 conference, which will take place on March 19th and 20th at the Zlatá Štika Hotel in Pardubice. The conference is organized by the Czech Society of Clinic...
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Kapras Day 2025
We would like to invite you to the 23rd Kapras Day on the topic of "Clinical Genetics," which will take place on Wednesday, February 26, 2025, in the Congress Hall of Hotel Olšanka in Prague. We loo...
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Description:
- Chemically competent E. coli cells with high stability suitable for high efficiency transformation. For research use only.
- Parent of DH10B/TOP10 and derived strains.
Application:
- For cloning, sub-cloning and phage display, but cannot be used for reinfection.
- Transform efficiency is >1.0x108 cfu/µg with pUC19 control DNA.
Genotype:
F- Δ(ara-leu)7697 [araD139]B/r Δ(codB-lacI)3 galK16 galE15 λ- e14- mcrA0 relA1 rpsL150(StrR) spoT1 mcrB1 hsdR2(r-m+)
Recommended storage condition:
This product should be stored at -80°C. Thaw on ice only before use. Do not re-freeze
Competent Cell Type:
Chemically
Transformation Efficiency (cfu/μg):
>1.0x108 cfu/µg with pUC19 control DNA
Blue-White Screening:
Yes, IPTG induction required
Strain:
K12
Reduces Recombination:
Yes
Cloning Methylated DNA:
Yes
Improves Plasmid Quality:
Yes
Preparing Unmethylated DNA:
Not suitable
T1 Phage Resistant:
No
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