
EPPiC Fast - Enzymatic PostPCR immediate Clean Up Fast
Quick and Effective PCR fragment purification
The EPPiC Fast mixture contains two enzymes that effectively degrade dNTPs and primers left-overs from previous PCR mixtures while remaining the double stranded DNA PCR products untouched. The EPPiC Fast enzymes are active at 37 °C in standard buffers used in PCR and are completely thermally inactivated by 1 min at 80 °C incubation.
The procedure takes only 6 minutes
The EPPiC Fast purifed PCR fragments are free from dNTPs and original PCR primers and may be directly used in downstream applications including nested, second round PCR, SNP analysis, molecular cloning and cycle sequencing reactions.
Features |
Specifications |
Form |
solution |
Size |
100 or 500 reactions in 10 ul |
Contents |
Recombined thermolabile nucleotide hydrolase Recombined exonuclease I |
Storage |
-20°C (we do not recommend freezing in - 80˚C because it can influence on activity of the mix) |
Applications |
sequencing, cloning, SNP analysis, PCR |