ngs ONCO anti-EGFR

Principle of detection

DNA samples are prepared in a two-step PCR amplification. Target hotspot regions are amplified during the 1st multiplex PCR reaction and sample specific molecular barcodes are added in the 2nd universal PCR amplification. Individual library samples are purified, normalized, pooled and sequenced on the Illumina MiSeq® platform. Sequencing data are processed and analyzed by ERMI software and the user can export a report. Free of charge use of the ERMI evaluation software is included in the kit. The software is very user-friendly and its use does not require any bioinformatic knowledge.

Parameters of the diagnostic kit

• Diagnostic kits for next-generation sequencing on Illumina MiSeq® platform
• Up to 64 samples per sequencing run (when used with v2 300 cycles chemistry)
• Panel of 8 relevant genes for molecular diagnostics of somatic mutations in colorectal cancer, lung cancer, and melanomas using the anti-EGFR antibody therapy
• Carefully designed primers for single-tube amplicon multiplex PCR library preparation providing uniform coverage
• Suitable with the FFPE samples and input DNA in amounts as low as 20 ng
• Straightforward library preparation protocol with short hands-on time
• Developed in collaboration with experts from the Clinic of Oncology, General University Hospital in Prague
• Bioinformatic software solution provides user friendly data analysis and generates a report