dCas13d

Adaptive immune systems from prokaryotes utilize clustered regularly interspaced short palindromic repeats (CRISPRs) and CRISPR associated (Cas) proteins to cleave foreign genetic material. CasRx, Cas13d from Ruminococcus flavefaciens XPD3002, is highly active and short, 94KD.

The dcas13a is a mutant cas13a. It can bind target RNA and can not cut the RNA. 

Applications

Targeted RNA knockdown

RNA editing

Nucleic acid detection

Source
E. coli

Organism
Ruminococcus flavefaciens XPD3002 (CasRx)

Molecule Weight: 94KD

UniProt #
U2PSH1

Purity
> 95 % as determined by SDS-PAGE

Storage
-20 Avoid repeated freeze-thaw cycles.

Cas13 Storage Buffer
0.3 M NaCl, 20 mM Tris-HCl, pH 8.0, 0.5 mM EDTA, 1 mM DTT, 50% Glycerol

10X Cas13 Reaction Buffer
200 mM HEPES, 50 mM MgCl₂, 1 M NaCl, 1 mM EDTA, pH 6.5

10X Cas13 sherlock reaction buffer (requested)
200 mM HEPES, 90 mM MgCl₂, pH 6.8

Name	Cat#	Description
dCas13d	dCAS13d-100	100 μg,1 mg/ml
dCas13d	dCAS13d-200	1 mg,1 mg/ml
dCas13d	dCAS13d-300	100 mg,10 mg/ml