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- Nucleic Acid Analysis
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Enzymes
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- Mesophilic DNA Polymerases
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News
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XXXV. Izakovičov memoriál 2025
We are pleased to announce our participation in the prestigious XXXV. Izakovič Memorial 2025, which will take place on October 8–10, 2025 at the Grandhotel Praha, Tatranská Lomnica. The Izakovič Memo...
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1st Czechoslovak Congress of Medical Genetics 2025
In the spring, we will participate in the 1st Czechoslovak Congress of Medical Genetics, which will take place from April 2–4, 2025, at the Cultural and Congress Center Elektra in the spa town of Luha...
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RANK 2025
Visit us at the 19th edition of the RANK 2025 conference, which will take place on March 19th and 20th at the Zlatá Štika Hotel in Pardubice. The conference is organized by the Czech Society of Clinic...
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Description
5´ DNA Adenylation Kit is for the enzymatic synthesis of 5’ adenylated ssDNA linkers. The kit is optimized to produce the adenylated DNA with or without 3´ terminator. The 5´ DNA adenylation kit can simply and efficiently generate greater than 95% conversion of pDNA to AppDNA.
Features
- One step reaction. Simple and efficient.
- 65°C reaction temperature reduces secondary structural concerns.
- Highly efficient process eliminates the need for purification of the product.
- Easily scale up from pmol to µmol range.
Application
Enzymatic 5´adenylation of single-stranded DNA linkers for next generation sequencing (NGS).
Kit Components
10X 5’ DNA Adenylation Reaction Buffer
500 mM Sodium Acetate (pH 6.0 @ 25°C)
100 mM MgCl2
50 mM DTT
1 mM EDTA
1 mM ATP
Mth RNA Ligase
Storage Temperature:-20°C
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