Blunting Express Kit

Description
The Blunting Express Kit can be used to generate blunt-ended DNA fragments for subsequent use in ligation, cloning, cDNA construction, and MCLABs Topomize Amplicon Library Prep Kit (Cat# TopoA-50A or TopoA-50B).  Our proprietary enzyme mix effectively fills in 5 overhangs and eliminates A-tailed 3 overhangs on the template. This Blunting Express Kit is optimized to provide a rapid workflow with only 2 minutes of incubation. Efficiency is combined with a high performance to streamline your blunt-end objectives.

Recommended Storage Condition: -20°C

Kit Components

10x Buffer

dNTP Mix (25mM)

Enzyme Mix

Figure

PCR fragment size checked before and after end blunting by the kit. Data are generated by Applied Biosystems 3730/3730xl DNA Analyzer.