RNase H, Bst

Description
RNase H is an endoribonuclease which degrades the RNA strand of RNA/DNA hybrid molecules. RNase H digestion produces ribonucleotide molecules with 5'-phosphate and 3'-hydroxyl termini. RNAse H is nearly inactive against single or double-stranded RNA molecules.
RNase H from the thermophilic bacterium Bacillus stearothermophilus is thermostable, has optimal activity above 65°C and making it useful for a broad range of applications.

Applications

Removal of poly(A) tails of mRNA hybridized to poly(dT)

Removal of mRNA during second strand cDNA synthesis

Source
A recombinant E. coli strain carrying the RNAse H gene from the thermophilic bacterium Bacillus stearothermophilus

Supplied in
20 mM Tris-HCl, 100 mM KCl, 10 mM MgCl_2, 0.1 mM EDTA, 0.1 mM DTT, 50% Glycerol, pH 7.9 @ 25°C

Supplied with
10x RNAse H Buffer

10x RNAse H Buffer
500 mM Tris-HCl, 750 mM KCl, 30 mM MgCl_2, 100 mM DTT, pH 8.3 @ 25°C

Unit Definition:
1 unit is defined as the amount of enzyme that will hydrolyze 1 nmol of RNA from an 3H-labeled DNA:RNA hybrid molecule into acid-soluble material in 20 minutes at 37°C.

Recommended Storage Condition: -20°C