Endonuclease V, E. coli

Description
Endonuclease V, (Endo V) is a 3'-endonuclease involved in DNA repair, which initiates removal of deaminated bases from damaged DNA, including uracil, hypoxanthine, and xanthine.

Endonuclease V is also active toward abasic sites and urea sites, base pair mismatches, flap and pseudo Y structures, and small insertions/deletions in DNA molecules. The cleavage site generated by Endonuclease V is at the second phosphodiester bond 3' to a lesion.

Applications
High-throughput methods for mutation research
Studies in mutagenesis and DNA repair
Mismatch cleavage
Genotyping

Source
E. coli

Purity
> 95 % as determined by SDS-PAGE

Storage
-80 Avoid repeated freeze-thaw cycles.

Unit Definition
One unit of T4 Endonuclease V converts 1 µg of UV-irradiated plasmid DNA from covalently closed circles to nicked form in 30 minutes at 37°C.