Description
Human Recombinant Alkyl Adenine DNA Glycosylase produced in E.Coli is a single, non-glycosylated polypeptide chain containing 306 amino acids (1-298 a.a.) and having a molecular mass of 33.9kDa (Molecular weight on SDS-PAGE will appear higher).
MPG is fused to an 8 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Applications
Single cell gel electrophoresis (Comet Assay)
Alkaline elution
Alkaline unwinding
Source
E. coli
Purity
> 95 % as determined by SDS-PAGE
Storage
-80 Avoid repeated freeze-thaw cycles.