MutS Protein (Thermus aquaticus)

Description
The MutS DNA mismatch protein recognizes heteroduplex DNAs containing mispaired or unpaired bases. This Muts protein binds in vitro to heteroduplex DNAs containing mispaired or unpaired bases over a wide temperature range from 4 to 70 °C and has a thermostable ATPase activity. This thermostable Taq MutS is active at temperature between 0 to 75°C. Since Taq MutS efficiently binds to 1-4 bases deletion (or insertion) and mismatch base pairs of GT, CT and AG, it is useful for detecting these mutations. Mutations can be detected in polyacrylamide gels or on a solid phase such as Ni agarose or beads or magnetic Ni-NTA particles.

Applications

Remove mismatch DNA (error correction) from gene synthesis reaction

Mutation detection and removal

Rapid isothermal SNP detection

Full name
Thermus aquaticus MutS DNA mismatch repair protein

Source

E. coli

Fusion Tag
6XHis tag at C-terminus

Accession #
AAC43637

Molecule Weight
92.8 kDa

Purity
> 98 % as determined by SDS-PAGE

Storage Buffer
20 mM Tris-HCl, pH 8.0, 250 mM NaCl, 0.1mM EDTA, 1 mM DTT, 50% Glycerol

Reaction Buffer
100mM KCl, 50 mM Tris-HCl, pH 8.5, 10 mM MgCl₂, 0.1 mM EDTA, 1 mM DTT, 2% Glycerol, 65 °C

Storage
-20°C Avoid repeated freeze-thaw cycles.