https://www.carolinabiosystems.cz/614-thickbox_default/bovine-enterokinase-rbek.jpg View full size

Bovine Enterokinase (rbEK)

Contact us

EK-100

Enterokinase (EK) is an enzyme produced by cells of the duodenum and involved in human digestion.

Description
Enterokinase (EK) is an enzyme produced by cells of the duodenum and involved in human digestion. It plays a role of turning trypsinogen to its active form trypsin, and indirectly activates the pancreatic digestive enzymes. Enterokinase is a specific protease that cleaves after a lysine preceded by four aspartic acids: Asp-Asp-Asp-Asp-Lys. Enterokinase will not work if the recognition site is followed by a proline.

 

Full Name
Enterokinase

 

Source
E. coli


Species
Bovine

 

Accession #
P98072

 

Molecule Weight
Recombinant Bovine Enterokinase (rbEK) as the light chain is a single glycosylated polypeptide chain with 235 a.a. and a 6-his tag. It has a predicted molecular mass of 27 kDa.

 

Purity
> 95 % as determined by SDS-PAGE

 

Endotoxin
< 1 EU per μg of the protein as determined by the LAL method

Unit Definition: One unit is defined as the amount of enzyme needed to cleave 50 μg of fusion protein in 16 hours to 95% completion at 22°C in a buffer containing 25mM Tris-HCl, pH 8.0.

 

Biological Activity
100 IU/μg

 

Storage
rbEK remains stable up to 1 year at -20°C from date of receipt. It will remain stable at 37°C for one week without losing any activity. Please avoid freeze-thaw cycles. Avoid repeated freeze-thaw cycles.

Cart  

No products

0,00 € Total

Cart Checkout

Payment gate

Platební loga