IDA Agarose for Phosphoproteins & Phosphopeptides

Description

FastSep high performance resins and chromatography cartridges

Numerous ligand-metal ion combinations 
High affinity & high capacity 
Minimal non-specific binding & excellent purity 
Long spacer arms & minimal steric hindrance 
Low metal ion leaching & excellent stability 
Maintain high performance after multiple repeated use 

Application

For phosphoprotein & phosphopeptide purification 
Simply replace your existing NTA resin or cartridge, no optimization or protocol changes necessary 
Purification under native and denaturing conditions 
Suitable for small proteins, large protein complexes, and proteins with low expression rates 

Purification of Phosphoproteins & Phosphopeptides

Immobilized Al3+, Ga3+, and Fe3+ are the most widely used ions for selective enrichment of phosphopeptides from complex proteolytic digest mixtures containing both phosphorylated and nonphosphorylated components. The most commonly used ligands are NTA and IDA. Depending on identity of proteins, impurity profile and purification conditions, different ligand-metal ion combinations can achieve different purification results. 

How to choose different ligands and metal ions in IMAC?

NTA and IDA are the two most widely used ligands in IMAC. NTA is a tetravalent ligand with coordination number 4 and therefore stronger coordination of metal ions, while IDA is a trivalent ligand with coordination number 3. Generally, NTA gives higher binding specificity and lower metal ion leaching, while IDA shows more non-specific binding and increased metal ion leaching. IDA can have higher metal ion loading density and usually requires much lower imidazole concentration in eluent. 

As many factors can affect the choice of optimal ligand-metal ion combination, the best approach is to try different combinations and let your proteins decide. FastSep(TM) high performance resins and cartridges offer numerous ligand-metal ion combinations to meet various purification needs. 

FastSep(TM) high performance resins and chromatography cartridges are based on highly cross-linked agarose 6% beads which have structural characteristics that result in superb physical strength, excellent flow properties, low backpressure, and an open pore structure. FastSep(TM) high performance resins and chromatography cartridges allow operation at very high flow rates, resulting in increased throughput and rapid column cleaning between chromatography runs.

Specification