Description
Triton X-100 is a commonly used detergent in laboratories[1]. For example:
Permeabilize unfixed (or lightly fixed) eukaryotic cell membranes.
Used in conjunction with zwitterionic detergents such as CHAPS to solubilize membrane proteins in their native state.
Used in DNA extraction as part of the lysis buffer (usually in a 5% solution in alkaline lysis buffer).
Reduce surface tension of aqueous solutions during immunostaining (usually in concentration of 0.1-0.5% in TBS or PBS Buffer).
Emerging use in dispersion of carbon materials for soft composite materials
Used in microbiology to restrict colony expansion in Aspergillus nidulans.