5M Betain

Description
5M solution of betaine in MilliQ H2O. DNase, RNase free.

Betaine is a PCR enhancing agent that has been used successfully for increasing yield and specificity of PCR products. It equalizes the contribution of GC- and AT-base pairing to the stability of the DNA duplex. Therefore, it's very useful to GC-rich PCR fragment amplification.

Usage
Use as a 5x stock solution. The final betaine concentration in the PCR reaction is 1M. 
For example, add 5 ul 5M betaine to 25 ul PCR reaction or 10 ul to 50 ul PCR reaction.

Application: PCR Reagent