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Bovine Serum Albumin (BSA) Acetylated

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BSA-100

Acetylated BSA is used as a carrier protein and an enzyme stabilizer in which the absence of contaminants such as nucleases and proteases is vital. The acetylation process inactivates any trace of active nuclease activity. While the acetylation process does change BSA's binding characteristics and makes it inhibitory in PCR reactions, it is normally added to restriction digests.

Description
Acetylated BSA is used as a carrier protein and as an enzyme stabilizer in which the absence of contaminants such as nucleases and proteases is vital. The acetylation process inactivates any trace of active nuclease activity. While the acetylation process does change BSA's binding characteristics and makes it inhibitory in PCR reactions, it is normally added to restriction digests.

 

Applications

Used as a blocking agent in Northern, Southern and dot blot hybridizations

Added to buffers for nick translation, polymerase reactions and ligations

Common additive for PCR amplifications, footprinting and gel shift assays

Helps stabilize some proteins during incubation

Enhances enzymes activity in restriction digests

Concentration
20 mg/ml

 

Recommended Storage Condition:
BSA should be stored at –20 °C. Do not store in a frost-free freezer.

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