Glutathione Agarose Cartridge

Reference: GAB-CC1x5

Glutathione S-transferase (GST) gene fusion systems have been widely used for obtaining large amounts of desirable protein in Escherichia coli.

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Description:
Glutathione S-transferase (GST) gene fusion systems have been widely used for obtaining large amounts of desirable protein in Escherichia coli. The fusion protein, which contains a GST tail can then be purified through affinity chromatography. MCLAB’s glutathione agarose is designed for the specific purification of GST recombinant proteins and other glutathione-binding proteins. Glutathione agarose is uniquely formulated for excellent binding capacity and purity of the protein of interest.

Specifications

Chromatography technique	GST-tagged protein purification
Matrix	Highly cross-linked 6% beaded agarose
Active group	Glutathione
Active group density	>40 umol/ml drained medium
Spacer	1,4-bis(2,3-epoxypropoxy)butane (12 atom stable and uncharged ether hydrophilic linkage)
Bead geometry & size	Spherical 50 to 150 um
Bead mean diameter d50v	90 um
Linear flow velocity	<75 cm/h at 25 °C, HR 16/10 column, 5 cm bed height
Recommended linear flow rate	<30 cm/h
Pressure/flow specification	Base matrix 100-200 cm/h, pressure drop cm H2O/bed height = 15, bed height 10 cm, 5 cm i.d.
1ml Cartridge bed dimensions	7×25 mm
1ml Cartridge bed height	25 mm
1ml Cartridge bed volume	1 ml
1ml Cartridge column I.D	7 mm
5ml Cartridge bed dimensions	16×25 mm
5ml Cartridge bed height	25 mm
5ml Cartridge bed volume	5 ml
5ml Cartridge column I.D	16 mm
Maximum cartridge flow rate	5 ml/min (1 ml cartridge) or 20 ml/min (5ml cartridge)
Recommended cartridge flow rate	1 ml/min (1 ml cartridge) or 5 ml/min (5 ml cartridge)
Maximum pressure during operation	5 bar [0.5 MPa] (70 psi)
pH Stability working range	3 to 13
pH Stability cleaning in place (cip)	2 to 14
Chemical stability	Stable to commonly used aqueous solutions. Can be used with non-ionic detergents, denaturing solvents, e.g. 8 M urea and 6 M guanidine hydrochloride, Stable in organic solvents, e.g. 50% methylformamide and 50% dioxane.
Storage	2 to 8 °C