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SP Cross-linked Agarose Big Beads

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SPS-100

SP Cross-linked Agarose Big Beads are strong ion exchangers designed for industrial applications. The large particle sizes (100-300 µm) and excellent physical stability of the base matrix ensure maintained speed even with viscous samples.

Description
SP Cross-linked Agarose Big Beads are strong ion exchangers designed for industrial applications. The large particle sizes (100-300 µm) and excellent physical stability of the base matrix ensures maintained speed, even with viscous samples. SP Cross-linked Agarose Big Beads are therefore the ultimate ion exchange media for initial purifications when high viscosity precludes the use of ion exchangers with smaller bead size, such as Cross-linked Agarose Fast Flow ion exchangers. The unique flow characteristics are also invaluable when adsorption needs to be done quickly.

Features

Easy to scale-up

High flow rates

High chemical resistance for effective cleaning-in-place (CIP)

Easy maintenance

 

Specification

Ion exchanger type Sulfopropyl strong cation
Ionic capacity 0.18-0.25 mmol (H+)/ml
Dynamic capacity 70 mg RNase/ml medium
Pressure/flow spec. 1200-1800 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm
Average particle size 200 μm (100-300 μm)
Matrix Highly cross-linked agarose, 6%
pH stability 3-14 (short term), 4-12 (long term)
Chemical stability Stable in all common aqueous buffers:
       8 M urea, 6 M guanidine HCl, 70% ethanol, 1 M NaOH*, and 1 M acetic acid*.
Storage 20% ethanol (Q, DEAE, ANX, CM), 0.2 M sodium acetate in 20% ethanol (SP)
Storage temperature 4°C to 30°C

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