Gel-Out

Description:
Gel-Out is a kit for DNA extraction from agarose gel using minicolumns. It processes agarose blocks of up to 200 mg and is suited for DNA amounts from 2 to 20 µg. Electrophoresis can be performed in either TAE or TBE buffer. The excised gel slice is dissolved with R7SI solution, which contains a color pH indicator. A yellow mixture confirms the optimal pH for DNA binding to the silica membrane, while a pink color signals too high a pH, which can be corrected by adding the sodium acetate supplied in the kit. After the addition of isopropanol, the DNA is bound to the minicolumn, washed with A1 wash solution and eluted with TE buffer or sterile water.

Features:

• DNA extraction from agarose gel on minicolumns
• R7SI agarose melting solution with a color pH indicator
• Sodium acetate for pH adjustment included
• Binding capacity 20 µg DNA, minimum DNA amount 2 µg
• DNA fragment size range 100–10,000 bp
• Typical recovery 60–80%
• Sample size: agarose block up to 200 mg
• Elution volume 30–50 µl
• Elution solution: TE buffer or water (water not included)
• Suitable for gels prepared in TAE or TBE buffer
• For research use only

Applications:

• DNA extraction from agarose gel after electrophoresis: excised gel slice up to 200 mg
• Isolation of DNA amounts from 2 to 20 µg. For smaller amounts the Gel-Out Concentrator kit is more suitable

Kit contents:

• Minicolumns
• R7SI agarose melting solution
• A1 wash solution
• Sodium acetate (3 M, pH 5.5)
• Isopropanol
• TE buffer

Specification: