Clean-Up Maxi

Description:
Clean-Up Maxi is a kit for cleanup of large amounts of DNA after PCR and other enzymatic reactions, for example with restriction enzymes, ligase or kinase. The column capacity is 1 mg of DNA and the kit processes samples of up to 10 ml. The sample is mixed with two volumes of G binding solution and applied to the column placed in a 50 ml tube. If the volume of the mixture exceeds 25 ml, it is divided between several columns. The DNA is then washed with A1 wash solution and eluted in 5 ml of Tris buffer, TE buffer or sterile water, which is recommended to be preheated to 70 °C.

Features:

• DNA cleanup on maxicolumns
• Binding capacity 1000 µg (1 mg) DNA
• Sample size up to 10 ml
• Maximum mixture volume per column 25 ml
• Elution volume 5 ml
• Elution solution: Tris buffer (included), TE buffer or water (not included)
• Counterweight column for centrifugation included
• A swing-out rotor for 50 ml tubes is required for centrifugation (not included)
• For research use only

Applications:

• Cleanup of larger amounts of DNA after PCR
• Cleanup of larger amounts of DNA after enzymatic reactions: with restriction enzymes, ligase, kinase and other enzymes

Kit contents:

• Columns
• 50 ml tubes
• Counterweight column
• G binding solution
• A1 wash solution
• Tris buffer (10 mM, pH 8.5)

Specification: