PvuII (reduced star activity)

Source: Proteus vulgaris

5’-C A G C T G-3’
3’-G T C G A C-5’

Purified from an E.coli strain carrying the cloned and purified PvuII gene from Proteus vulgaris.

Reaction Temperature: 37°C

Prototype: PvuII

Inactivation Temperature (20 min): —

Reaction Buffer:

1x ONE Buffer

Unit Definition: One unit is the amount of enzyme required to completely digest 1 µg of lambda DNA in 1 hr in a total reaction volume of 50 µl.

To avoid star activity it is not recommended to incubate over night.

Storage Conditions: Store at –20°C.

Quality Control:

All preparations are assayed for contaminating endonuclease, 3′-exonuclease, 5′-exonuclease/5′-phosphatase, as well as non-specific single- and double-stranded DNase activities.