MCNext™ DNA Sample Prep Kit

Description

The MCNext DNA Sample Prep Kit is a high performance, cost-effective sample prep solution for NGS using the Illumina platforms. A bead-based, gel-free size selection workflow in only 90 min eliminates the need for mechanical fragmentation and agarose gels in size selection, the availability of pair-ends 96 indices facilitates multiplex high-throughput applications. The MCNext DNA Sample Prep Kit incorporates different flanking sequence additions by Dialatum™ transposome, resulting in fewer primer-dimers and a better signal/noise ratio in sequencing outcomes.

Fast Prep Workflow

• Complete the protocol in 90 minutes
• Enzymatic fragmentation without sonication
• One-step fragmentation/tagging to save time  

Low Input and High Throughput

• Maximum of 50 ng input DNA needed
• Pre-mixed enzymes and nucleotides for multiplexed processing
• Plate-based processing compatible for up to 96 samples per kit

Higher Coverage Rate and Full Compatibility

• Directional transposome doubles the sequencing coverage
• Works on all Illumina NGS platforms
• All 96 Illumina indices included

Figure 1. MCNext™ Sample Preparation Kit workflow takes 90 min to finish the sample prep.

Figure 2. MCNext™ Sample Preparation Kit fragments and directionally tags DNA in a single step to boost sequencing coverage and signal strength. Subsequent limited-cycle PCR amplification adds dual indices to enable up to 96 libraries to be pooled and sequenced.