Description
The MCMag PCR purification system utilizes solid-phase reversible immobilization (SPRI) paramagnetic bead technology for PCR amplicon purification. The MCMag beads are pre-formulated with an optimized buffer to selectively bind DNA fragments of 100 bp and larger. Excess salts, enzymes, primers and nucleotides can be removed through a simple washing procedure. The MCMag PCR Purification system is fully adaptable to automation.
Applications
Material Supplied
Specifications
Table 1. Available product sizes
MCMag PCR Purification |
Catalog Number |
5 mL |
MCPP-5 |
60 mL |
MCPP-60 |
450 mL |
MCPP-450 |
Table 2. Number of PCR reactions purified with 96- and 384-well formats
Product Size |
|||
PCR reaction volume (µL) 96-well format |
5 mL |
60 mL |
450 mL |
10 |
278 rxns |
3332 rxns |
25000 rxns |
20 |
139 rxns |
1666 rxns |
12500 rxns |
50 |
56 rxns |
667 rxns |
5000 rxns |
100 |
28 rxns |
334 rxns |
2500 rxns |
PCR reaction volume (µL) 384-well format |
|||
5 |
556 rxns |
6667 rxns |
50000 rxns |
7 |
397 rxns |
4762 rxns |
35714 rxns |
10 |
278 rxns |
3333 rxns |
20000 rxns |
14 |
198 rxns |
2381 rxns |
17857 rxns |
Materials Not Supplied