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MCMag PCR Purification Kit

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MCPP-5

Description
The MCMag PCR purification system utilizes solid-phase reversible immobilization (SPRI) paramagnetic bead technology for PCR amplicon purification. The MCMag beads are pre-formulated with an optimized buffer to selectively bind DNA fragments of 100 bp and larger. Excess salts, enzymes, primers and nucleotides can be removed through a simple washing procedure. The MCMag PCR Purification system is fully adaptable to automation.

Applications

  • PCR
  • Sequencing
  • Fragment Analysis
  • Genotyping
  • Cloning
  • Primer Walking

Material Supplied

  • MCMag PCR Purification beads
  • Store at 4°C upon arrival, for up to 18 months
  • Shake the reagent well to a homogenous appearance before use

Specifications

Table 1. Available product sizes

MCMag PCR Purification

Catalog Number

5 mL

MCPP-5

60 mL

MCPP-60

450 mL

MCPP-450

 

Table 2. Number of PCR reactions purified with 96- and 384-well formats

 

Product Size

PCR reaction volume (µL)  96-well format

5 mL

60 mL

450 mL

10

278 rxns

3332 rxns

25000 rxns

20

139 rxns

1666 rxns

12500 rxns

50

56 rxns

667 rxns

5000 rxns

100

28 rxns

334 rxns

2500 rxns

PCR reaction volume (µL)   384-well format

     

5

556 rxns

6667 rxns

50000 rxns

7

397 rxns

4762 rxns

35714 rxns

10

278 rxns

3333 rxns

20000 rxns

14

198 rxns

2381 rxns

17857 rxns

 

Materials Not Supplied

  • Reaction Plate
  • McBead Magnetic Plate
  • Plate Seals, Adhesive or Heat
  • Wash Solution (70% ethanol)
  • Elution Buffer
    • Water
    • Tris-Acetate (10 mM pH 8.0)
    • TE Buffer (10 mM Tris-Acetate pH 8.0, 1 mM EDTA

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