
In the spring, we will participate in the 1st Czechoslovak Congress of Medical Genetics, which will take place from April 2–4, 2025, at the Cultural and Congress Center Elektra in the spa town of Luha...
Read moreVisit us at the 19th edition of the RANK 2025 conference, which will take place on March 19th and 20th at the Zlatá Štika Hotel in Pardubice. The conference is organized by the Czech Society of Clinic...
Read moreWe would like to invite you to the 23rd Kapras Day on the topic of "Clinical Genetics," which will take place on Wednesday, February 26, 2025, in the Congress Hall of Hotel Olšanka in Prague. We loo...
Read moreDescription:
QuantumScript™ HD Reverse Transcriptase is an engineered version with increased sensitivity, improved specificity and maximum thermostability. QuantumScript™ HD Reverse Transcriptase has been engineered to have longer half life at 50°C, which enables its ability to process longer RNA with more complexed secondary structures. Enhanced thermostability of this enzyme is obtained through re-engineered RNA-based DNA Polymerase domain and the fusion of a novel RNA-interacting surface domain at the RNase H domain site. The enzyme is purified to homogeneity to ensure high thermostability, specificity, fidelity, yield, and more full length cDNA synthesis that the premium reverse transcriptase provides. The optimal fist-strand cDNA synthesis temperature for this enzyme is 50°C, and it has a broad working temperature range from 37° C to 55° C, with cDNA product size from 100 bp to 12 Kb.
Catalog No.
SSIII-100, SSIII-200 and SSIII-300
Source
E.coli
Concentration
200 u/μl
Storage Buffer
20 mM Tris-HCl (pH 7.5), 1 mM DTT, 0.05% (v/v)Triton X-100, 0.1 mM EDTA, 0.1 M NaCl and 50% (v/v) glycerol.
Reaction Buffer (5x)
250 mM Tris-HCl (pH 8.3), 375 mM KCl, 15 mM MgCl2, and 50 mM DTT
Unit Definition
One unit of the enzyme incorporates 1 nmole of dTTP into acid-precipitable material in 10 minutes at 37˚C using poly (A):oligo (dT)25 as template-primer.
Quality Control
This enzyme has passed the quality control assays: SDS-PAGE analysis for purity, functional absence of endonuclease activities, functional absence of exonuclease activities, functional absence of protease activity.
Storage and Handling: -20° C
Protocol
First-Strand cDNA Synthesis
Materials to Be Supplied by the User
The following procedure uses 10 pg to 5 µg of total RNA or 10 pg to 500 ng of mRNA.
Note: The 5X Reaction Buffer is compatible with enzymes used in a number of downstream applications. Typically there is no need for phenol extractions or ethanol precipitations using this protocol before any PCR amplification.