GT XDetector

Description: 

The GT XDetector Kit is an advanced genetic tool designed for profiling female samples in complex forensic and kinship cases. It contains 17 STR markers, 15 of which are specific to the X chromosome, organized into four linkage groups. This kit enables accurate identification of female genotypes even in the presence of male samples and is ideal for linkage analysis when a disease gene is located on the X chromosome.

Features:

• Easy to use – ready-to-use mixture
• Multiplex analysis – analysis of 17 loci in a single reaction
• Sample mix-up detection – includes an autosomal marker
• Specific primers – produce sharp peaks without non-specific signals
• Wide application – can be used on various DNA sources such as blood, hair, bones, saliva, or directly on a sample using a DNA banking card (DBC®) or DBC Blue®
• Direct amplification – allows direct amplification from blood, amniotic fluid, cultured cells, chorionic villi, and other tissues using lysis buffers (GT BLB, GT AFLB, GT CVLB)

Markers:

The image below illustrates the markers contained in the GT XDetector kit, which are specific to the X chromosome. The markers are divided into four linkage groups, color-coded as blue, green, yellow, and red. At the bottom, the GT500 Size Standard is displayed, which ensures accurate fragment size calibration during DNA analysis.

This graphical overview facilitates orientation among markers and their linkage groups, which is crucial for the effective use of the kit in genetic analysis.

Chromosomal Locations:

The table below shows the GT XDetector Kit markers, displaying names, dye used, size range, and chromosome locations.

The image shows the precise locations of STR markers on the X chromosome included in the GT XDetector kit. The markers are color-coded into linkage groups and localized according to cytogenetic regions. This overview provides a visual guide for effective genetic linkage analysis and identification of specific regions on the X chromosome.

GT Detector Global Allelic Ladder:

The graph presents electropherograms obtained from marker analysis using the GT XDetector kit in capillary electrophoresis. The profiles contain sharp peaks of markers, divided by color into blue, green, yellow, and red, corresponding to the respective linkage groups of STR markers on the X chromosome. Each profile indicates the exact DNA fragment sizes within a specific range, enabling reliable genotype analysis.

The graph demonstrates the high quality and accuracy of results obtained with the kit, confirming its efficiency in forensic and kinship DNA analysis.

Compatibility and Usage:

The kit is compatible with Applied Biosystems™ 3500/xL, 3130/xl, and SeqStudio platforms for detection and analysis. The GT XDetector kit has been tested on more than 400 samples. It can be used on DNA extracted from hair roots, bone DNA, or directly in PCR without extraction (e.g., using GT BLB or GT DBC/GT DBC Blue filter papers).

Kit Contents:

The kit contains all necessary reagents and buffers for multiplex PCR (Multiplex Primer mix, PCR buffer, GT HSTaq DNA Pol in Box A). Additionally, the kit includes the GT XDetector Allelic Ladder, GT500 Size Standard, and GTM5 Matrix Standard for genetic analyzer calibration (Box B).

Package Sizes:

50 reactions (Cat. No. GT-12201-50)
100 reactions (Cat. No. GT-12201-100)
1,000 reactions (Cat. No. GT-12201-1K)

Comparison Chart of HID Kits: A comparative graph of Genetek Biopharma’s HID (Human Identification) kits used for genetic analysis. The graph illustrates the number of genetic markers detected by each kit, categorized by marker type.