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- Nucleic Acid Analysis
- Protein Analysis
- Biochemical Reagents
-
Enzymes
- Thermophilic DNA Polymerases
- Mesophilic DNA Polymerases
- Restriction Endonucleases
- Reverse Transcriptase and RNA Polymerases
- DNA/RNA Ligases
- RNases
- Proteases
- Nucleases
- Kinases
- Phosphatases and Sulfurylases
- DNA Repair Proteins
- Single-Stranded DNA Binding Proteins
- Chaperon Proteins and Disulfide Bond Isomerase
- Others
- Gene editing
- Molecular cloning
- Clinical diagnostics
- Human Identification STR kits
- Laboratory instruments
- Software
- A&A Biotechnology
- AdvancedSeq
- BioDynami
News
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1st Czechoslovak Congress of Medical Genetics 2025
In the spring, we will participate in the 1st Czechoslovak Congress of Medical Genetics, which will take place from April 2–4, 2025, at the Cultural and Congress Center Elektra in the spa town of Luha...
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RANK 2025
Visit us at the 19th edition of the RANK 2025 conference, which will take place on March 19th and 20th at the Zlatá Štika Hotel in Pardubice. The conference is organized by the Czech Society of Clinic...
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Kapras Day 2025
We would like to invite you to the 23rd Kapras Day on the topic of "Clinical Genetics," which will take place on Wednesday, February 26, 2025, in the Congress Hall of Hotel Olšanka in Prague. We loo...
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PRODUCT HIGHLIGHTS
- DNA Removing columns for efficient
separation of DNA and cellular debris
- No need for organic extractions
- No need for time-consuming alcohol
precipitations
- Immediate inactivation of endogenous
and exogenous RNases
- Efficient separation of enzyme
inhibitors and contaminants
- Final elution with RNase free water
PRINCIPLE
Erythrocytes in the sample are efficiently
lysed and the leucocytes separated
by centrifugation. After lysis under
denaturing conditions and simultaneous
inactivation of RNases the lysate is
transferred to a PerfectBind column.
RNA binds to the column´s silica
matrix and can be washed with
specific buffers to efficiently remove
contaminants like hemoglobin. The RNA
is finally eluted with RNase free water.
PERFORMANCE BLOOD RNA KIT
- Protocol time: < 60 min
- Column volume: 0.75 ml
- Elution volume: 50 – 100 μl
- Binding capacity: 100 μg/column
- RNA yield: 1 – 5 μg from
1 ml blood
- For up to 1 ml blood or up to
150 μl frozen blood/column
COLUMN OPTIONS
- Safety Line columns with snap-on lids
- Classic Line columns without lids
KIT COMPONENTS
- PerfectBind RNA columns
(Safety or Classic Line)
- Collection Tubes
- 'DNA Removing' columns
- Lysis Buffer ER
- RNA Lysis Buffer T
- RNA Wash Buffer I and II
- RNase free water
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