NTRK Gene Fusions Detection Kit

Description:

AmoyDx^® NTRK Gene Fusions Detection Kit is a real-time PCR assay. This kit enables the qualitative detection of up to 109 fusions involving the NTRK1/2/3 genes in human RNA extracted from solid tumor formalin-fixed paraffin-ded (FFPE) tissue samples. It is designed to assist in identifying NTRK fusion status for patients who may benefit from approved targeted therapies. The kit is intended for in vitro diagnostic use and should be operated by trained professionals in a laboratory setting.

Principle:

The kit adopts Amplification Refractory Mutation System (ARMS) and real-time PCR technology, which comprises specific primers and fluorescent probes to detect NTRK mutations in human genomic DNA. During the nucleic acid amplification, the targeted mutant DNA is matched with the bases at 3’ end of the primer, amplified selectively and efficiently, then the mutant amplicon is detected by fluorescent probes labeled with FAM. While the wild-type DNA cannot be matched with specific primers, there is no amplification occurs.

High sensitivity: up to 1%
 

Detected fusions:

Tests/kit: 24 (bulk)

Instrument: Stratagene Mx3000P™, ABI 7500, ABI 7900HT, ABI StepOnePlus, LightCycler480 II, Bio-Rad CFX96, SLAN-96S