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- Nucleic Acid Analysis
- Protein Analysis
- Biochemical Reagents
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Enzymes
- Thermophilic DNA Polymerases
- Mesophilic DNA Polymerases
- Restriction Endonucleases
- Reverse Transcriptase and RNA Polymerases
- DNA/RNA Ligases
- RNases
- Proteases
- Nucleases
- Kinases
- Phosphatases and Sulfurylases
- DNA Repair Proteins
- Single-Stranded DNA Binding Proteins
- Chaperon Proteins and Disulfide Bond Isomerase
- Others
- Gene editing
- Molecular cloning
- Clinical diagnostics
- Human Identification STR kits
- Laboratory instruments
- Software
- A&A Biotechnology
- AdvancedSeq
- BioDynami
- Plant Cell Technology
News
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XXXV. Izakovičov memoriál 2025
We are pleased to announce our participation in the prestigious XXXV. Izakovič Memorial 2025, which will take place on October 8–10, 2025 at the Grandhotel Praha, Tatranská Lomnica. The Izakovič Memo...
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1st Czechoslovak Congress of Medical Genetics 2025
In the spring, we will participate in the 1st Czechoslovak Congress of Medical Genetics, which will take place from April 2–4, 2025, at the Cultural and Congress Center Elektra in the spa town of Luha...
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RANK 2025
Visit us at the 19th edition of the RANK 2025 conference, which will take place on March 19th and 20th at the Zlatá Štika Hotel in Pardubice. The conference is organized by the Czech Society of Clinic...
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Description
MCNext™ UT DNA Sample Prep Kit provides a fast and easy workflow to prepare up to 96 pooled, indexed paired-end libraries from low input amplicons (>400bp), microbial genomes and plasmids for the subsequent cluster generation and DNA sequencing using Illumina® NGS sequencing instruments.
The goal of this protocol is to fragmentate and add adapter sequences onto the template DNA with a single tube reaction to generate multiplexed single read or paired-end sequencing libraries.
Features
Fast Prep Workflow
- Complete protocol in 110 minutes
- Enzymatic fragmentation without sonication
- One-step fragmentation/tagging to save time
Low Input and High Throughput
- Only 1 ng input DNA needed
- Pre-mixed enzymes and nucleotides for multiplexed processing
- Plate-based processing compatible for up to 96 samples per kit
Higher Coverage Rate and Full Compatibility
- Directional transposome doubles the sequencing coverage
- Works on all the Illumina NGS platforms
Best Value on Market
- Most complete kit with all reagents included
- All 96 Illumina indices included
- Standardized library quantification prior to sample pooling and sequencing
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