MCNext™ UT DNA Sample Prep Kit

Description

MCNext™ UT DNA Sample Prep Kit provides a fast and easy workflow to prepare up to 96 pooled, indexed paired-end libraries from low input amplicons (>400bp), microbial genomes and plasmids for the subsequent cluster generation and DNA sequencing using   Illumina® NGS sequencing instruments.
The goal of this protocol is to fragmentate and add adapter sequences onto the template DNA with a single tube reaction to generate multiplexed single read or paired-end sequencing libraries.

Features

Fast Prep Workflow

• Complete protocol in 110 minutes
• Enzymatic fragmentation without sonication
• One-step fragmentation/tagging to save time 

Low Input and High Throughput

• Only 1 ng input DNA needed
• Pre-mixed enzymes and nucleotides for multiplexed processing
• Plate-based processing compatible for up to 96 samples per kit

Higher Coverage Rate and Full Compatibility

• Directional transposome doubles the sequencing coverage
• Works on all the Illumina NGS platforms

Best Value on Market

• Most complete kit with all reagents included
• All 96 Illumina indices included
• Standardized library quantification prior to sample pooling and sequencing