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- Nucleic Acid Analysis
- Protein Analysis
- Biochemical Reagents
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Enzymes
- Thermophilic DNA Polymerases
- Mesophilic DNA Polymerases
- Restriction Endonucleases
- Reverse Transcriptase and RNA Polymerases
- DNA/RNA Ligases
- RNases
- Proteases
- Nucleases
- Kinases
- Phosphatases and Sulfurylases
- DNA Repair Proteins
- Single-Stranded DNA Binding Proteins
- Chaperon Proteins and Disulfide Bond Isomerase
- Others
- Gene editing
- Molecular cloning
- Clinical diagnostics
- Human Identification STR kits
- Laboratory instruments
- Software
- A&A Biotechnology
News
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1st Czechoslovak Congress of Medical Genetics 2025
In the spring, we will participate in the 1st Czechoslovak Congress of Medical Genetics, which will take place from April 2–4, 2025, at the Cultural and Congress Center Elektra in the spa town of Luha...
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RANK 2025
Visit us at the 19th edition of the RANK 2025 conference, which will take place on March 19th and 20th at the Zlatá Štika Hotel in Pardubice. The conference is organized by the Czech Society of Clinic...
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Kapras Day 2025
We would like to invite you to the 23rd Kapras Day on the topic of "Clinical Genetics," which will take place on Wednesday, February 26, 2025, in the Congress Hall of Hotel Olšanka in Prague. We loo...
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| Description: MCLAB's RNA-Seq Library Construction Kit is a highly efficient library construction kit for preparing single, paired-end or multiplexed cDNA libraries for high-throughput sequencing. This kit can be used to convert RNA transcripts into cDNA whole transcriptome libraries or small RNA libraries for next-generation sequencing analysis using Illumina® GAIIxTM, HiSeqTM 2000 and MiSeq® sequencing platforms. With preserving the complexity of sequencing libraries, the protocol is quicker than the standard method and could be used routinely for RNA sequencing through the Illumina®platform. Function: The superior function of MCLAB's RNA-Seq Library Construction Kit depends on our proprietary enzyme systems. Unique improvement to each key enzyme at specific steps in the library construction workflow increases sensitivity, flexibility and speed for next-generation sequencing. The target RNA fragments can be tagged with linkers at both ends by RNA ligases (Cat# T4RL1-100 and T4RL2T-100). Corresponding cDNA can be synthesized by Universal Reverse Transcriptase (Cat# SSII-100). The cDNA library can be enriched by PCR with high fidelity Pfu DNA Polymerase (Cat# AD-200). After gel- or beads-based purification for size selection, final cDNA libraries can hybridize directly to the oligonucleotides on the flow cell surface for cluster generation and sequencing thereafter. Features: - Simplified workflow, reducing hands-on time - Optimized reaction conditions, increasing sensitivity - Straightforward protocol minimizing prior experience needed - Strand-specific sequencing data - Cost-effective with all key enzymes - High flexibility: multiplex samples and automation friendly - Functionally validated with Illumina® GAIIxTM, HiSeqTM 2000 and MiSeq® sequencing platforms Recommended Storage Conditions: -20°C. |
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