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  • 1st Czechoslovak Congress of Medical Genetics 2025

    In the spring, we will participate in the 1st Czechoslovak Congress of Medical Genetics, which will take place from April 2–4, 2025, at the Cultural and Congress Center Elektra in the spa town of Luha...

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  • RANK 2025

    Visit us at the 19th edition of the RANK 2025 conference, which will take place on March 19th and 20th at the Zlatá Štika Hotel in Pardubice. The conference is organized by the Czech Society of Clinic...

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  • Kapras Day 2025

    We would like to invite you to the 23rd Kapras Day on the topic of  "Clinical Genetics," which will take place on Wednesday, February 26, 2025, in the Congress Hall of Hotel Olšanka in Prague. We loo...

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T4 DNA Polymerase

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T4DP-100

Description:
T4 DNA Polymerase catalyzes the extension of a primed DNA template in the 5' -> 3' direction. This enzyme exhibits a powerful 3' -> 5' exonuclease activity, while lacking any inherent 5' -> 3' exonuclease or strand displacement functions.

Specific Activity: 5,555U/mg

Application:
- 3'-overhang removal to form blunt ends
- 5'-overhang fill-in to form blunt ends
- Single strand deletion for sub-cloning
- Second strand synthesis in site-directed mutagenesis
- Probe labeling using replacement synthesis

Source:
Purified from a strain of E. coli that expresses the recombinant T4 DNA Polymerase gene.

Supplied in:
100 mM KPO4
1.0 mM DTT
0.1 mM EDTA
50% Glycerol
pH 6.5 @ 25°C

Supplied With: 10x Blue Buffer

10x Blue Buffer:
500 mM NaCl
100 mM Tris-HCl
100 mM MgCl2
10 mM DTT
pH 7.9 @ 25°C

Unit Definition:
One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTPs into acid-precipitable material in 30 minutes at 37°C.

Recommended Storage Condition: -20ºC

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