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EB Buffer
Buffer EB is the elution buffer used in many Qiagen kits, e.g. PCR, Gel Extraction, Nucleotide Removal Kits, MinElute Kits and the QIAprep Miniprep Kits for DNA cleanup or small-scale plasmid purification. The purified DNA can also be eluted in TE (10 mM Tris-Cl, 1 mM EDTA, pH 8.0), but the EDTA may inhibit certain subsequent enzymatic reactions.
30% Brij-35
Brij-35 (30% Solution) is a nonionic polyoxyethylene surfactant that is most frequently used as a component of cell lysis buffers or as a surfactant in various HPLC applications.
MutS Protein (Thermus aquaticus)
The MutS DNA mismatch protein recognizes heteroduplex DNAs containing mispaired or unpaired bases.
RNase H, Bst
RNase H is an endoribonuclease which degrades the RNA strand of RNA/DNA hybrid molecules.
Blunting Express Kit
The Blunting Express Kit can be used to generate blunt-ended DNA fragments for subsequent use in ligation, cloning, cDNA construction, and MCLAB’s Topomize Amplicon Library Prep Kit (Cat# TopoA-50A or TopoA-50B). Our proprietary enzyme mix effectively fills in 5’ overhangs and eliminates A-tailed 3’ overhangs on the template.
