MC Lab (9)

CHAPS (3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate ) is a zwitterionic detergent used to solubilize proteins. It is used as a non-denaturing solvent in the process of protein purification and is especially useful in purifying membrane proteins.

EDTA, 0.5 M, pH 8.0, is a sterile-filtered solution of 0.5 M EDTA in H₂O treated with diethyl pyrocarbonate (DEPC).

RNase H is an endoribonuclease which degrades the RNA strand of RNA/DNA hybrid molecules.

Terminal deoxynucleotidyl transferase (TdT) is a template-independent DNA polymerase that catalyzes the addition of deoxynucleotides to the 3’ hydroxyl terminus of single or double stranded DNA molecules.

The 2X MCAmp library amplification master mix has been specially formulated to provide accurate and robust amplification of NGS (next-generation sequencing) library fragments.

1 mg, 1 mg/ml

5,000 units, 20,000 U/ml

2,500 units,10,000 U/ml

ROX Free, for Real-time PCR Machines BioRad iCycler MiniOpticon, Opticon 2, Chromo4, iQ5; Roche LightCycler 480; MJ Research DNA Engine Opticon 2, Chromo4; Corbett Roto-gene 3000, 6000, 4x1.25ml

synthesis at higher temperatures

reduced RNase H activity

addition of deoxynucleotides to the 3′ hydroxyl terminus

origin: E.coli (gen from Z. albicollis)

purity > 95 %

TWEEN 20 helps to prevent non-specific antibody binding in enzyme immunoassay as a washing agent (e.g. Western blots and ELISAs). In this major application, it is dissolved in Tris-Buffered Saline or Phosphate buffered saline at dilutions of 0.05% to 0.5% v/v.

D-(+)-Glucose is a common natural sugar involved in processes such as energy production, glycosylation, and formation of glycans that provide structure to cells. Glucose is involved in a detrimental process in cells called glycation. Glucose is used as a supplement for cell culture and in numerous cellular processes and molecular biology applications.

Benzonase endonuclease from Serratia marcescens, can be used to degrade all forms of DNA and RNA while having no proteolytic activity.

MCMax Cell-Free DNA Extraction Kit is designed to purify cell-free DNA (cfDNA) from 0.2–10 ml plasma samples. The kit contains everything necessary for purification, with no preprocessing required. The purified cell-free DNA is of high quality with little genomic DNA contamination and is ready for use in downstream applications such as qPCR, next-generation sequencing and digital PCR.

10 μg, 100 ug/ml

CHAPSO (3-[(3-cholamidopropyl)dimethylammonio]-2-hydroxy-1-propanesulfonate) is a zwitterionic detergent used to solubilize proteins. Due to its low light absorbance in the ultraviolet region of the electromagnetic spectrum, CHAPSO is useful for researchers monitoring ongoing chemical reactions or protein-protein binding with UV/Vis spectroscopy.